Composite
Part:BBa_K3823012:Design
Designed by: Yiying Zhang Group: iGEM21_Tongji_China (2021-10-01)
hydrogen sulfide sensitive, three-gear adjustable kill switch
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1300
Illegal PstI site found at 919
Illegal PstI site found at 1607 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1300
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 919
Illegal PstI site found at 1607 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1300
Illegal BglII site found at 896
Illegal BglII site found at 2482
Illegal BamHI site found at 36
Illegal BamHI site found at 543
Illegal BamHI site found at 1660
Illegal BamHI site found at 2996
Illegal XhoI site found at 2954 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1300
Illegal PstI site found at 919
Illegal PstI site found at 1607 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1300
Illegal PstI site found at 919
Illegal PstI site found at 1607 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
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