Composite

Part:BBa_K3823012:Design

Designed by: Yiying Zhang   Group: iGEM21_Tongji_China   (2021-10-01)


hydrogen sulfide sensitive, three-gear adjustable kill switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1300
    Illegal PstI site found at 919
    Illegal PstI site found at 1607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1300
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 919
    Illegal PstI site found at 1607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1300
    Illegal BglII site found at 896
    Illegal BglII site found at 2482
    Illegal BamHI site found at 36
    Illegal BamHI site found at 543
    Illegal BamHI site found at 1660
    Illegal BamHI site found at 2996
    Illegal XhoI site found at 2954
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1300
    Illegal PstI site found at 919
    Illegal PstI site found at 1607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1300
    Illegal PstI site found at 919
    Illegal PstI site found at 1607
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References